Electrically Conductive and 3D?Printable Oxidized Alginate?Gelatin Polypyrrole:PSS Hydrogels for Tissue Engineering

Electroactive hydrogels can be used to influence cell response and maturation by electrical stimulation. However, hydrogel formulations which are 3D printable, electroactive, cytocompatible, allow adhesion, remain a challenge in the design of such stimuli-responsive biomaterials for tissue engineering. Here, combination pyrrole with high gelatin-content oxidized alginate-gelatin (ADA-GEL) is reported, offering 3D-printability precursors prepare cytocompatible electrically conductive scaffolds. By oxidation pyrrole, electroactive polypyrrole:polystyrenesulfonate (PPy:PSS) synthesized inside ADA-GEL matrix. The assessed regarding their electrical/mechanical properties, 3D-printability, cytocompatibility. It possible open-porous scaffolds via bioplotting have higher seeding efficiency scaffold depth comparison flat 2D hydrogels, confirmed multiphoton fluorescence microscopy. formation an interpenetrating polypyrrole matrix increases conductivity stiffness maintaining capacity gels promote adhesion proliferation. results demonstrate that 3D-printable rendered (ADA-GEL-PPy:PSS), promise therapies, vitro culture, electrical-stimulation assisted